Restriction digests of the clone give the following sizes (kb): BamHI--8.0, 2.6; EcoRI--5.7, 5.0; HindIII--10.5; SalI--10.5; HincII--3.7, 3.3, 1.9, 1.05, 0.85.
A shuttle vector which replicates as a stable, nonintegrated, multicopy plasmid in transformed mouse cells and in Escherichia coli.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Sarver N, et al. Transformation and replication in mouse cells of a bovine papillomavirus-pML2 plasmid vector that can be rescued in bacteria. Proc. Natl. Acad. Sci. USA 79: 7147-7151, 1982. PubMed: 6296820
