Restriction digests of the clone give the following sizes (kb): EcoRI--12.0; HindIII--8.0, 2.9; SalI--12.0; BamHI--8.5, 3.1; PstI--12.0.
Light emission can be detected after promoter insertion by adding dodecyl aldehyde.
A promoter cloning, Escherichia coli/Bacillus subtilis shuttle vector.
Media
ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37.0°C
References
Carmi OA, et al. Use of bacterial luciferase to establish a promoter probe vehicle capable of nondestructive real-time analysis of gene expression in bacillus spp. J. Bacteriol. 169: 2165-2170, 1987. PubMed: 3106332
