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pGMB

規格:

價格:

編號:B241657

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pGMB

商品貨號

B241657

Designations

pGMB

GenBank Number

X52259

培養基

Species

Homo sapiens, human

Depositors

ML Lane

Vector

Construct size (kb): 3.984999895095825

Insert

DNA: genomic

Insert lengths(kb): 1.241999983787537

Tissue: DNA from NIH 3T3 cells transformed by MCF7 DNA

Gene product: sec oncogene [SEC*]

Insert Size (kb)

1.242

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.6, 1.3; EcoRI--3.9; HindIII--3.9; PstI--3.9; HincII--3.9.

The insert begins 133 nucleotides 5' to nucleotide 1 of the published sequence and extends 520 nucleotides 3' to nucleotide 540 of the published sequence.

The insert is oriented such that transcription from the SP6 promoter gives the sense strand and from the T7 promoter gives the antisense strand.

References

Lane MA, et al. Nucleotide sequence of a human oncogene active in tumors of secretory epithelium. Nucleic Acids Res. 18: 3068, 1990. PubMed: 2349114

Lane MA, et al. Activation of related transforming genes in mouse and human mammary carcinomas. Proc. Natl. Acad. Sci. USA 78: 5185-5189, 1981. PubMed: 6272293

Mary Ann L Lane, personal communication

B239056：pGMB

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