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AKAP100
AKAP100
規格:
價格:
編號:B241670
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
AKAP100
商品貨號
B241670
Designations
AKAP100
GenBank Number

U17195

培養基
Species
Homo sapiens, human
Depositors
JD Scott
Applications
in suitable host, produces protein A-kinase anchor protein 100
Vector
Construct size (kb): 7.639999866485596
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pET11d-kfc
Intact vector size: 5.800
Type of vector: plasmid
Vector end: NcoI
Vector end: BamHI
Cloning sites: NcoI BamHI
Polylinker sites:
Construction: pET11d
Host range: Escherichia coli
Features (with orientation and position when available):
transcription terminator: T7, <-
other: calmodulin binding domain, <-
restriction site: KpnI
restriction site: BglII
other: factor X unit, <-
restriction site: SalI
other: kemptide unit, <-
restriction site: BamHI
epitope tag: T7-tag coding sequence, <-
restriction site: NcoI
promoter for in vitro transcription: T7, <-
repressor gene: lacI, ->
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: AKAP100*
Gene name: A-kinase anchor protein 100
Contains complete coding sequence?: Y
Tissue: hippocampus
Type of DNA: cDNA
Insert 5' end: NcoI
Insert 3' end: BamHI
Insert size (kb): 1.965
Cross references: DNA Seq. Acc.: U17195
Nucleotides 1-1965 of the insert correspond to
nucleotides 1-1965 of U17195.
Insert lengths(kb): 1.9650000333786
Tissue: hippocampus
Gene product: A-kinase anchor protein 100 [AKAP100*]
Target Gene: A-kinase anchor protein 100
Insert Size (kb)
1.965
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/NcoI--5.8, 2.0; EcoRI--6.0, 1.8; HindIII--7.0, 0.6; PstI--6.2, 1.6; PvuII--3.0, 2.8, 1.5, 0.3.
Constructed by ligating a PCR product creating an NcoI site on the 5' end to a fragment from a cDNA library.
The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--0.60; HindIII--1.75; NdeI--0.67; PstI--1.55; PvuII--1.31, 1.62; SacI--0.09.
Transcription from the T7 promoter allows expression of a kfc-fusion protein that can be purified by affinity chromatography on calmodulin-agarose. (kfc refers to kemptide - factor X - calmodulin-binding domains).
References

McCartney S, et al. Cloning and characterization of A-kinase anchor protein 100 (AKAP100). A protein that targets A-kinase to the sarcoplasmic reticulum. J. Biol. Chem. 270: 9327-9333, 1995. PubMed: 7721854

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