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pVH17

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編號:B241693

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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產品名稱

pVH17

商品貨號

B241693

Designations

pVH17

GenBank Number

X03224

培養基

Species

Escherichia coli (Migula) Castellani and Chalmers

Depositors

C Wong

Applications

produces protein 2-deoxyribose-5-phosphate aldolase

Vector

Construct size (kb): 5.069999217987061

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: EcoRI
Vector end: AvaI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749

Insert

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Escherichia coli
Gene symbol: deoC
Gene name: 2-deoxyribose-5-phosphate aldolase
Contains complete coding sequence?: Y
Type of DNA: genomic
Insert 5' end: EcoRI
Insert 3' end: AvaI
Insert size (kb): 2.13
Cross references: DNA Seq. Acc.: X03224

Insert lengths(kb): 2.130000114440918

Gene product: 2-deoxyribose-5-phosphate aldolase [deoC]

Target Gene: 2-deoxyribose-5-phosphate aldolase

Insert Size (kb)

2.130

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--4.5, 0.5; BglII--5.0; EcoRI/AvaI--3.0, 1.6, 0.5. The 0.5 kb EcoRI and the 1.6 kb AvaI/EcoRI fragment are from the insert.

The insert contains the following restriction sites (approximate kb from the 5' end): ClaI--0.46, 0.53; EcoRI--0.48; BglII--0.59.

Contains the two tandem promoter/operator regions for the deo operon, the complete coding sequence for deoC, and 53 bp of deoA. The insert includes approximately 350 bp 5' sequence not in the GenBank record.

Classification

Enterobacteriaceae, Escherichia

References

Valentin-Hansen P, et al. The structure of tandem regulatory regions in the deo operon of Escherichia coli K12. EMBO J. 1: 317-322, 1982.

Chen L, et al. Deoxyribose-5-phosphate aldolase as a catalyst in asymmetric aldol condensation. J. Am. Chem. Soc. 114: 741-748, 1992.

Valentin-Hansen P, et al. The primary structure of Escherichia coli K12 2-deoxyribose 5-phosphate aldolase. Nucleotide sequence of the deoC gene and the amino acid sequence of the enzyme. Eur. J. Biochem. 125: 561-566, 1982. PubMed: 6749498

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