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pRIL6C.94
pRIL6C.94
規(guī)格:
價格:
編號:B242015
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pRIL6C.94
商品貨號
B242015
Designations
pRIL6C.94
GenBank Number

M26744

培養(yǎng)基
Species
Rattus norvegicus, rat
Depositors
GH Fey
Applications
Linearize the plasmid with PstI and use the T7 promoter for sense-strand mRNA synthesis.
A 0.9 kb BamHI-PstI fragment of the probe was used to detect a genomic BglI fragment of 13 kb in the rat and genomic BglI fragments of 23 and 6.2 kb in the mouse.
Vector
Construct size (kb): 3.859999895095825
Insert
DNA: cDNA
Insert lengths(kb): 0.9010000228881836
Tissue: peritoneal exudate cell line
Gene product: interleukin 6 (interferon, beta 2)(interleukin 6) [IL6]
Insert Size (kb)
0.901
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/PstI--3.1, 1.0; PstI--3.8; BamHI--3.8.
Linearize the plasmid with PstI and use the T7 promoter for sense-strand mRNA synthesis. Linearize with BamHI and use the T3 promoter for antisense-strand synthesis.
IL6 has been mapped to the rat chromosome 4.
This clone has the same insert as pRIL6C.91 (which was constructed in a modified Okayama and Berg vector).
The insert contains nt 13 (XhoI site) through 923 (SspI site) of the full-length rat IL6 cDNA (start ATG is nt 65, stop TAG is nt 698). The mature protein is 187 amino acids, from nt 137 to nt 697.
The sequence for this clone is in the GenBank/EMBL data bank with accession numbers M26744 and M26745.
The derived rat amino acid sequence is 93% identical to mature murine IL6 and 58% identical to mature human IL6.
A 0.9 kb BamHI-PstI fragment of the probe was used to detect a genomic BglI fragment of 13 kb in the rat and genomic BglI fragments of 23 and 6.2 kb in the mouse.
References

Northemann W, et al. Structure of the rat interleukin 6 gene and its expression in macrophage-derived cells. J. Biol. Chem. 264: 16072-16082, 1989. PubMed: 2789217

Szpirer J, et al. The Interleukin-6-dependent DNA-binding protein gene (transcription factor 5: TCF5) maps to human chromosome 20 and rat chromosome 3, the IL6 receptor locus (IL6R) to human chromosome 1 and rat chromosome 2, and the rat IL6 gene to rat chromosome 4. Genomics 10: 539-546, 1991. PubMed: 1889804

Georg H Fey, personal communication

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