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Simian virus 40
Simian virus 40
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B242028
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Simian virus 40
商品貨號(hào)
B242028
Classification
Polyomaviridae, Polyomavirus
Agent
Simian virus 40
培養(yǎng)基
Strain
pUCSV40-B2E [molecular clone of Simian virus 40, SV-40]
Common Name
Simian virus 40
Applications
contains sequence
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Contains a full-length genome of SV40-B2 cloned into pUC-19 at the EcoRI site. SV40-B2 was isolated and cloned from a low-passage stock of SV40-Baylor that had been isolated from a contaminated poliovaccine.
Product Format
frozen
Shipping Information
?Frozen glycerol stock of Escherichia coli containing the plasmid.
Storage Conditions
Store unopened frozen vial at -80°C.
Comments
This variant of SV-40-Baylor has a non-archetypal viral regulatory region and is the commonly studied version of SV-40-Baylor. Faster growing than SV-40-B1 in BSC-1, Vero, CV-1, and TC-7 cells.
Contains a full-length genome of SV40-B2 cloned into pUC-19 at the EcoRI site. SV40-B2 was isolated and cloned from a low-passage stock of SV40-Baylor that had been isolated from a contaminated poliovaccine.
Growth Medium
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Recommended Host
E. coli
Growth Conditions
Temperature: 37.0°C
Name of Depositor
JA Lednicky, JS Butel
Special Collection
NCRR Contract
References

Lednicky JA, Butel JS. Tissue culture adaptation of natural isolates of simian virus 40: changes occur in viral regulatory region but not in carboxy-terminal domain of large T-antigen. J. Gen. Virol. 78: 1697-1705, 1997. PubMed: 9225047

Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955

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