| 產品名稱 |
Simian virus 40 |
| 商品貨號 |
B242028 |
| Classification |
Polyomaviridae, Polyomavirus |
| Agent |
Simian virus 40 |
| Strain |
pUCSV40-B2E [molecular clone of Simian virus 40, SV-40] |
| Common Name |
Simian virus 40 |
| Applications |
contains sequence |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
Contains a full-length genome of SV40-B2 cloned into pUC-19 at the EcoRI site. SV40-B2 was isolated and cloned from a low-passage stock of SV40-Baylor that had been isolated from a contaminated poliovaccine. |
| Product Format |
frozen |
| Shipping Information |
?Frozen glycerol stock of Escherichia coli containing the plasmid. |
| Storage Conditions |
Store unopened frozen vial at -80°C. |
| Comments |
This variant of SV-40-Baylor has a non-archetypal viral regulatory region and is the commonly studied version of SV-40-Baylor. Faster growing than SV-40-B1 in BSC-1, Vero, CV-1, and TC-7 cells. Contains a full-length genome of SV40-B2 cloned into pUC-19 at the EcoRI site. SV40-B2 was isolated and cloned from a low-passage stock of SV40-Baylor that had been isolated from a contaminated poliovaccine. |
| Growth Medium |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Recommended Host |
E. coli |
| Growth Conditions |
Temperature: 37.0°C |
| Name of Depositor |
JA Lednicky, JS Butel |
| Special Collection |
NCRR Contract |
| References |
Lednicky JA, Butel JS. Tissue culture adaptation of natural isolates of simian virus 40: changes occur in viral regulatory region but not in carboxy-terminal domain of large T-antigen. J. Gen. Virol. 78: 1697-1705, 1997. PubMed: 9225047
Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955
|
