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FN771

規(guī)格:

價格:

編號:B242202

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

FN771

商品貨號

B242202

Designations

FN771

GenBank Number

M10905

培養(yǎng)基

Species

Homo sapiens, human

Depositors

M Chu

Applications

The coding sequence includes part of the fibrin binding domain, the 3' region showing species-specific variation, and a conserved cysteine.

Vector

Construct size (kb): 5.699999809265137

Insert

DNA: cDNA

Insert lengths(kb): 1.299999952316284

Tissue: fibroblast ATCC CRL 1106 cell line

Gene product: fibronectin 1 [FN1]

Alleles: A2, E2, A1, B2, D2, B1, D1, E1

Insert Size (kb)

1.300

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

The insert contains the following restriction sites (number): EcoRI (1), HindIII (1), HaeII (1).

Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 1.0, 0.3; HindIII--5.7; PstI--5.7; EcoRI/HindIII--4.3, 0.9, 0.3, 0.2; BamHI--5.7.

The insert encodes the 3' 500 bp of the coding sequence and 3' untranslated sequences. The coding sequence includes part of the fibrin binding domain, the 3' region showing species-specific variation, and a conserved cysteine.

This probe detects a mRNA of 7.8 kb in human fibroblasts.

References

Bernard MP, et al. Human cellular fibronectin: comparison of the carboxyl-terminal portion with rat identifies primary structural domains separated by hypervariable regions. Biochemistry 24: 2698-2704, 1985. PubMed: 2992573

B218473：FN771

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