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當前位置: 首頁 > ATCC代理 > pCETP.11 [CEPT.10, lambdaCEPT.10, pCETP.11A] B242244
pCETP.11 [CEPT.10, lambdaCEPT.10, pCETP.11A]
pCETP.11 [CEPT.10, lambdaCEPT.10, pCETP.11A]
規格:
價格:
編號:B242244
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pCETP.11 [CEPT.10, lambdaCEPT.10, pCETP.11A]
商品貨號
B242244
Designations
pCETP.11 [CEPT.10, lambdaCEPT.10, pCETP.11A]
Species
Homo sapiens, human
培養基
Depositors
DT Drayna
Vector
Construct size (kb): 4.699999809265137
Insert
DNA: cDNA
Insert lengths(kb): 1.580000042915344
Tissue: liver
Gene product: cholesteryl ester transfer protein, plasma [CETP]
Alleles: C1, C2, A1, S1, A2, B1, B2, E1, E2, S2
Insert Size (kb)
1.580
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.7; BglII--3.2, 1.1; PvuII--2.4, 1.0, 0.54, 0.36; NdeI--4.5; BglI--3.9, 0.47.
Codominant segregation of both TaqI polymorphisms in 4 families (29 individuals). TaqIA and TaqIB polymorphisms exhibit a large degree of linkage equilibrium; no recombination between the two has been shown.
Contains the CETP coding sequence from amino acid 9 of the mature protein through the 3' untranslated sequence, ending with 17 residues of polyA.
Four haplotypes with frequencies 0.06 to 0.44.
S1/S2 alleles are difficult to detect with the entire probe. A 732 bp PstI/PvuII fragment detects the same polymorphism and the 3.4 kb invariant band.
TaqIA RFLP hybridizes much more strongly than the TaqIB RFLP. The MspI RFLP is quite sensitive to incomplete digestion.
Enzyme(s) not detecting polymorphism: BclI, BglII, BstEII, EcoRI, EcoRV, HindIII, PstI, PvuII and SacI.
Enzyme(s) not detecting polymorphism: ApaLI, Asp718, BglII, BsmI, HincII, NcoI, NdeI, PstI, SacI, ScaI, SphI, StuI (in 6 unrelated individuals).
References

Drayna D, et al. Cloning and sequencing of human cholesteryl ester transfer protein cDNA. Nature 327: 632-634, 1987. PubMed: 3600759

Drayna D, Lawn R. Multiple RFLPs at the human cholesteryl ester transfer protein (CETP) locus. Nucleic Acids Res. 15: 4698, 1987. PubMed: 2884631

Lusis AJ, et al. Assignment of the human gene for cholesteryl ester transfer protein to chromosome 16q12-16q21. Genomics 1: 232-235, 1987. PubMed: 3446583

Freeman D, et al. An StuI RFLP at the human cholesteryl ester transfer protein (CETP) locus. Nucleic Acids Res. 17: 2880, 1989. PubMed: 2566155

Julier C, et al. A primary map of 24 loci on human chromosome 16. Genomics 6: 419-427, 1990. PubMed: 2328986

Kinzler KW, Vogelstein B. EcoRI polymorphism within the GLI gene (chromosome 12q13.3-14.1). Nucleic Acids Res. 18: 2834, 1990. PubMed: 1971098

Dennis T Drayna, personal communication

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