| Insert |
DNA: genomic Insert lengths(kb): 13.30000019073486 Tissue: F8 cell line (T-prolymphocytic leukemia) Gene product: T cell receptor, gamma, variable region V3 [TCRGJ1] |
| Comments |
Restriction digests of the clone give the following sizes (kb): KpnI--17.0, 11.0, 4.8, 3.6, 1.8 (doublet), 1.5; PvuI--22.0, 13.0, 7.8; SacI--20.0, 9.1, 4.4 (doublet), 4.1; SmaI--20.0, 11.0, 6.2, 5.8; XhoI--20.0, 14.0, 9.1. The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--3.3, 5.1, 10.5. Contains a productive rearrangement of Vgamma3 and Jgamma1. The insert begins about 0.5 kb upstream of pseudogene Vgamma1 and extends 3.6 kb beyond J1. Additional GenBank/EMBL accession numbers: M12953, M13429. |
| References |
LeFranc MP, et al. Diversity and rearrangement of the human T cell rearranging gamma genes: nine germ-line variable genes belonging to two subgroups. Cell 45: 237-246, 1986. PubMed: 2938743
Marie-Paule Lefranc, personal communication
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