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pSV2-neo plasmid in <em>Escherichia coli</em>

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價格:

編號:B242301

品牌:Mingzhoubio

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定量菌液

DNA

RNA

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凍干粉

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產品名稱

pSV2-neo plasmid in Escherichia coli

商品貨號

B242301

Designations

pSV2-neo plasmid in Escherichia coli

Depositors

P Berg, Stanford University Medical Center

培養基

Biosafety Level

Host

Escherichia coli HB101

Vector Information

Size (kb): 5.729

Vector: pSV2-neo (plasmid)

Promoters: Promoter SV40 early

Construction: SV40, Tn5, pBR322

Marker(s): G418R,ampR

Construct size (kb): 5.729

Features: marker(s): G418R
marker(s): ampR
promoter: SV40 early
replicon: SV40
replicon: pMB1
restriction site: HindIII

Applications

Expression vector

Shuttle vector

Comments

Restriction digests of the clone give the following sizes (kb): PvuII--4.2, 0.74, 0.61, 0.36; BamHI--5.8; EcoRI--5.8; PstI--2.45, 1.40, 0.94 (doublet).

Provides dominant selectable marker for resistance to antibiotic G418 in mammalian cell lines.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37°C

References

Southern PJ, Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Appl. Genet. 1: 327-341, 1982. PubMed: 6286831

Shipped

frozen

Shipping Information

Glycerol stock of ?E. coli containing the plasmid

B239996：EBO-pSV2-neo

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