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BL29-113
BL29-113
規格:
價格:
編號:B242429
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
BL29-113
商品貨號
B242429
Designations
BL29-113
GenBank Number

T25885

培養基
Species
Homo sapiens, human
Depositors
R Sanders
Vector
Construct size (kb): 3.72000002861023
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: DNA Segment, numerous copies, expressed
Contains complete coding sequence?: U
Tissue: BL29 cell line
Disease: Burkitt's lymphoma
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 0.82
Cross references: DNA Seq. Acc.: T25885; EST: EST113R; Primer: T3
Insert lengths(kb): 0.8199999928474426
Tissue: BL29 cell line Burkitt's lymphoma
Disease: Burkitt's lymphoma
Gene product: DNA Segment, numerous copies, expressed
Insert Size (kb)
0.820
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PvuII--2.5, 0.86, 0.34; PstI--3.3, 0.45; HindIII--3.7; EcoRI--2.9, 0.6, 0.24; BamHI--3.7.
Maps to 8 different chromosomes.
References

Rhiannon Sanders, personal communication

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