ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.1, 0.96; PstI--3.3, 0.80; HindIII--4.0; PvuII--2.6, 1.45; AccI--3.2, 0.78.
The insert contains the following restriction sites (approximate kb from the 5' end): AccI--0.18; PstI--0.79.
Contains 5' and 3' untranslated regions and the complete coding sequence.
The insert is oriented such that transcription from the T3 promoter gives the sense strand.
References
Lopez JA, et al. The alpha and beta chains of human platlet glycoprotein Ib are both transmembrane proteins containing a leucine-rich amino acid sequence. Proc. Natl. Acad. Sci. USA 85: 2135-2139, 1988. PubMed: 3353370
