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pBD64
pBD64
規格:
價格:
編號:B242594
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pBD64
商品貨號
B242594
Designations
pBD64
Depositors
D Dubnau
培養基
Biosafety Level
1
Vector Information
Size (kb): 4.6999998092651370
Vector: pBD64 (plasmid)
Construction: pUB110, pC194
Marker(s):cmlR,kanR
Construct size (kb): 4.699999809265137
Features: marker(s): cmlR, kanR
replicon: pUB110
Applications
shuttle vector
Comments
A general purpose plasmid vector for cloning in Bacillus subtilis.
This vector was isolated as a spontaneous deletion variant of pBD12, a chimeric vector composed of plasmids pUB110 and pC194. The deletion consists of 1844 bp (nt 3803 to 5649) of pBD12, and encompasses nt 1 to 487 and 1552 to 2906 of the pC194 sequence.
Media
ATCC® Medium 1179: VY medium
Growth Conditions
Temperature: 37.0°C
References

Gryczan T, et al. Characterization of chimeric plasmid cloning vehicles in Bacillus subtilis. J. Bacteriol. 141: 246-253, 1980. PubMed: 6243622

Minton NP, et al. The gram-positive cloning vector pBD64 arose by a 1844 bp deletion of pC194 derived DNA. Nucleic Acids Res. 18: 1651, 1990. PubMed: 2326208

Shipped
freeze-dried
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