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pRed-227
pRed-227
規(guī)格:
價格:
編號:B242607
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pRed-227
商品貨號
B242607
Designations
pRed-227
Species
Cricetus cricetus, hamster
培養(yǎng)基
Depositors
KL Luskey
Vector
Construct size (kb): 7.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 4.5
Tissue: CHO UT1 cell line
Gene product: 3-hydroxy-3-methylglutaryl-Coenzyme A reductase [HMGCR]
Insert Size (kb)
4.5
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): SalI--7.4; BamHI--4.37, 2.7, 0.3.
A full-length clone which includes the entire coding region, 163 bp of 5'-untranslated sequence and 1.65 kb of 3'-untranslated sequence. It codes for a protein of 887 amino acids.
Hybridizes to genomic EcoRI fragments of the following sizes: mouse 3T3 (14.5, 2.1 kb), Chinese hamster (10.1, 3.3, 2.8, 1.45 kb), and rat (9.1, 7.7, 3.2 kb).
References

Simonet WS, Ness GC. Post-transcriptional regulation of 3-hydroxy-3-methylglutaryl-CoA reductase mRNA in rat liver. Glucocorticoids bloc the stabilization caused by thyroid hormones. J. Biol. Chem. 264: 569-573, 1989. PubMed: 2909540

Simonet WS, Ness GC. Transcriptional and posttranscriptional regulation of rat hepatic 3-hydroxy-3-methylglutaryl-coenzyme A reductase by thyroid hormones. J. Biol. Chem. 263: 12448-12453, 1988. PubMed: 3410847

Sundaresan S, et al. Genes for HMG-CoA reductase and serotonin 1a receptor are on mouse chromosome 13. Somatic Cell Mol. Genet. 15: 465-469, 1989. PubMed: 2781417

Chin DJ, et al. Nucleotide sequence of 3-hydroxy-3-methyl-glutaryl coenzyme A reductase, a glycoprotein of endoplasmic reticulum. Nature 308: 613-617, 1984. PubMed: 6546784

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