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pPD39
pPD39
規格:
價格:
編號:B242617
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pPD39
商品貨號
B242617
Designations
pPD39
GenBank Number

U02043

培養基
Species
Homo sapiens, human
Depositors
PL Deininger
Vector
Construct size (kb): 3.299999952316284
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS+
Intact vector size: 2.964
Type of vector: phagemid
Vector end: BamHI
Vector end: BamHI
Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Genomic copy number: repetitive
Gene name: Alu consensus sequence, repetitive
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end: Modification: BamHI linkers
Insert end: Modification: BamHI linkers
Insert size (kb): 0.33
Cross references: DNA Seq. Acc.: U02043
Insert lengths(kb): 0.3300000131130219
Gene product: Alu consensus sequence, repetitive
Insert Size (kb)
0.330
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.0, 0.4; PvuI--2.3, 1.1; SacI--3.4; PvuII--2.6, 0.8; KpnI--3.4.
A perfect HS consensus sequence (HSC3N1) modified at the ends (4 bp and 24 bp) for cloning.
Hybridizes efficiently to a few Alu sequences in a mouse cell background at 52C in 50% formamide, with a wash at 61C. Because the sequence is closer to consensus than BLUR8, it matches rodent B1 family better with an increase in rodent background.
References

Batzer MA, et al. A consensus Alu repeat probe for physical mapping. GATA 11: 34-38, 1994. PubMed: 7917498

Prescott L Deininger, personal communication

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