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pCR1000DQB1*0607
pCR1000DQB1*0607
規格:
價格:
編號:B242623
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pCR1000DQB1*0607
商品貨號
B242623
Designations
pCR1000DQB1*0607
GenBank Number

M87041

培養基
Species
Homo sapiens, human
Depositors
L Baxter-Lowe
Vector
Construct size (kb): 3.269999980926514
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCR1000
Intact vector size: 3.000
Type of vector: plasmid
Vector end: T overhangs
Vector end: T overhangs
Cloning sites:
Polylinker sites: ApaI KpnI SacI SpeI PacI HindIII XmaI ins site NotI EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): kanR
promoter: lac, T7
replicon: colE1
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: HLA-DQB1
Other gene symbols: HLA-DQB
Genomic copy number: unique
Gene name: major histocompatibility complex, class II, DQ beta 1
Contains complete coding sequence?: U
Chromosome: 6; Localization: 6 p21.3
Tissue: peripheral blood leukocytes
Type of DNA: genomic
Insert end: Modification: A overhangs
Insert end: Modification: A overhangs
Insert size (kb): 0.27
Cross references: DNA Seq. Acc.: M87041
Insert lengths(kb): 0.2700000107288361
Tissue: peripheral blood leukocytes
Gene product: major histocompatibility complex, class II, DQ beta 1(major histocompatibility complex, class II, lymphocyte antigen DQB) [HLA-DQB]
Insert Size (kb)
0.270
Media
ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BglI--2.9, 0.38; SmaI--2.8, 0.4, 0.2; BamHI--uncut; EcoRI/HindIII--3.0, 0.32; HindIII--3.3.
The insert contains the following restriction sites (bp from the 5' end): BglI--129; SmaI--188.
Encodes the *0607 or the *06BRI1 allele, which differs from DQB1*0603 by a substitution at codon 57 [GTT (valine) replacing GAT (aspartic acid)].
References

Fenske TS, Baxter-Lowe LA. Two novel HLA-DQB1*06 alleles reveal additional heterogeneity of HLA-DQw1. Tissue Antigens 40: 49-52, 1992. PubMed: 1440562

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