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pTK11
pTK11
規(guī)格:
價格:
編號:B242670
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱
pTK11
商品貨號
B242670
Designations
pTK11
GenBank Number

K02581

培養(yǎng)基
Species
Homo sapiens, human
Depositors
PL Deininger
Applications
in another host, produces protein thymidine kinase 2, mitochondrial
Vector
Construct size (kb): 4.775000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcD
Intact vector size: 3.000
Type of vector: plasmid
Cloning sites: XhoI BamHI
Polylinker sites:
Construction: pBR322, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: SV40
marker(s): ampR
promoter: SV40 early, late
replicon: pMB1, SV40
terminator: SV40 late
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: TK1
Genomic copy number: unique
Gene name: thymidine kinase 1, soluble
Contains complete coding sequence?: Y
Chromosome: 17; Localization: 17 q23.2-q25.3
Type of DNA: cDNA
Insert 5' end: Modification: GC
Insert 3' end: Modification: AT
Insert size (kb): 1.42
Cross references: DNA Seq. Acc.: K02581
Insert lengths(kb): 1.419999957084656
Gene product: thymidine kinase 1, soluble [TK1]
Alleles: A1, A2, B1, B2, B3
Target Gene: thymidine kinase 2, mitochondrial
Insert Size (kb)
1.420
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 1.6, 0.23; BamHI/SmaI--3.25, 1.40, 0.3; BglI--2.9, 1.25, 0.6, 0.38; HindIII--3.4, 1.55; XhoI--3.25, 1.90.
The insert contains the following restriction sites (approximate kb from the 5' end): SmaI--0.11; BglI--0.31, 0.65; HindIII--0.96; BamHI--1.34.
Although the GenBank K02581 gives the gene symbol for this sequence as TK2, this is the cDNA derived from the genomic sequence M15205, the gene for thymidine kinase 1 (TK1).
Contains the complete coding sequence (nt 58-762).
A 1.25 kb SmaI/BamHI fragment has been used as a hybridization probe. It detects a 3.0 kb HindIII fragment in chickens.
References

Bradshaw HD Jr., Deininger PL. Human thymidine kinase gene: molecular cloning and nucleotide sequence of a cDNA expressible in mammalian cells. Mol. Cell. Biol. 4: 2316-2320, 1984. PubMed: 6549046

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