Vector Information
Size (kb): 5.3800001144409180
DESCRIPTION OF VECTOR:
Intact vector size: 5.380
Type of vector: plasmid
Cloning sites: BamHI mLuI BfrI NotI EclXI KspI Asp718 KpnI ApaI SalI XbaI
SacI SphI EcoRV NcoI HindIII
Polylinker sites: BamHI mLuI BfrI NotI EclXI KspI Asp718 KpnI ApaI SalI XbaI
SacI PstI SphI EcoRV NcoI StyI HindIII
Construction: pUC-BM20, proU (pJL32-3), rrnB (pTrc99A), pBR322
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR, ->, 84-1274
replicon: rop (copy number control), 1913-2104
replicon: pMB1, 2532-2532
marker(s): ampR, <-, 3291-4161
transcription terminator: rrnB T2, <-, 4263-4340
transcription terminator: rrnB T1, <-, 4422-4465
MCS: BamHI...HindIII, <-, 4749-4672
coding sequence: proV 5' sequence, <-, 4756-5065
promoter for expression: proU, <-, 5063-5366
Vector: pOSEX2 (plasmid)
Promoters: Promoter for expression proU
Construction: pUC-BM20, proU (pJL32-3), rrnB (pTrc99A), pBR322
Marker(s):ampR,tetR
Construct size (kb): 5.380000114440918
Features: marker(s): ampR
marker(s): tetR
promoter for expression: proU
replicon: pMB1
replicon: rop (copy number control)
MCS: BamHI...HindIII
transcription terminator: rrnB T1
transcription terminator: rrnB T2
coding sequence: proV 5' sequence
