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pEFD139.1 [RW-40]
pEFD139.1 [RW-40]
規(guī)格:
價(jià)格:
編號(hào):B242874
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pEFD139.1 [RW-40]
商品貨號(hào)
B242874
Designations
pEFD139.1 [RW-40]
Species
Homo sapiens, human
培養(yǎng)基
Vector
Construct size (kb): 4.400000095367432
Insert
DNA: genomic
Insert lengths(kb): 1.700000047683716
Gene product: DNA Segment, single copy [D4S163]
Insert Size (kb)
1.700
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 0.7; HindIII--4.4; PstI--4.4; BamHI--4.4; EcoRI/HindIII--2.7, 1.0, 0.7.
The invariant fragments are derived from the acrocentric chromosomes (13, 14, 21, and 22).
The entire plasmid was labeled by the random primer method. Repetitive sequences were blocked by preannealing the probe with human DNA. Hybridization was at 65C for 14 hr. Blot were washed 3 times in 0.5 X SSC (0.1% SDS, 65C).
Enzyme(s) not detecting polymorphism: PstI.
References

Altherr MR, et al. A highly polymorphic VNTR locus on the long arm of chromosome 4. Nucleic Acids Res. 19: 1168, 1991. PubMed: 1673566

Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872

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