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p304
p304
規(guī)格:
價格:
編號:B242948
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
p304
商品貨號
B242948
Designations
p304
GenBank Number

J00127

培養(yǎng)基
Species
Homo sapiens, human
Applications
The depositor notes that the fibrinogen clones (ATCC 59706-59710) are suitable for applications in gene mapping or involving nucleic acid hybridization, but they are not suitable starting material for expression in mammalian cells.
Vector
Construct size (kb): 4.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 2.0
Tissue: liver
Gene product: fibrinogen, A alpha polypeptide [FGA]
Alleles: A1, A2
Insert Size (kb)
2.0
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.2, 1.9, 0.4; XhoI--4.8; EcoRI/XhoI--2.1, 1.1, 0.98; PstI--3.3, 1.1; BglI--3.2, 1.2; EcoRI/HindIII--2.3, 1.9; NcoI--2.5, 1.9.
Insert encodes fibrinogen A alpha amino acids 1-625 with an initiating ATG in front of amino acid 1. The vector is an expression vector.
The depositor notes that the fibrinogen clones (ATCC 59706-59710) are suitable for applications in gene mapping or involving nucleic acid hybridization, but they are not suitable starting material for expression in mammalian cells.
The clones do not encode the signal peptide necessary for assembly and secretion and one is known to lack a single nucleotide in the coding region.
The ability of this probe to detect the TaqI polymorphism was verified by J. Murray, University of Iowa.
References

Chung DW, et al. Characterization of complementary deoxyribonucleic acid and genomic deoxyribonucleic acid for the beta-chain of human fibronogen. Biochemistry 22: 3244-3250, 1983. PubMed: 6688356

Lord ST. Expression of a cloned human fibrinogen cDNA in Escherichia coli: synthesis of an A alpha polypeptide. DNA 4: 33-38, 1985. PubMed: 3882374

Susan T Lord, personal communication

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