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pRSVgpt

規格:

價格:

編號:B242958

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pRSVgpt

商品貨號

B242958

Designations

pRSVgpt

Depositors

BH Howard

培養基

Other IDs

Genbank : M83240 Genbank sequence

Biosafety Level

Vector Information

Size (kb): 0E-16

Vector: pRSVgpt (plasmid)

Promoters: Promoter RSV LTR

Construction: pRSVcat, pSV2gpt

Marker(s):ampR,xgpt

Construct size (kb): 3.557

Features: marker(s): ampR, xgpt
promoter: RSV LTR
replicon: pMB1

Applications

shuttle vector

Comments

Escherichia coli/mammalian cell culture vector with selectable markers in both systems

Plasmid vector containing the Escherichia coli xanthine guanine phosphoribosyltransferase (gpt) gene as a selection marker and the RSV LTR promoter.

Plasmid has been shown to stably transfect several mammalian cell lines (Science 221: 551-553, 1983).

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

Gorman C, et al. High efficiency DNA-mediated transformation of primate cells. Science 221: 551-553, 1983. PubMed: 6306768

Shipped

freeze-dried

Shipping Information

Shipped: ?Freeze dried E. coli containing the plasmid

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