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2A1 [Blast 1 cDNA, pB2A1, pB2A1.12]
2A1 [Blast 1 cDNA, pB2A1, pB2A1.12]
規格:
價格:
編號:B242973
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
2A1 [Blast 1 cDNA, pB2A1, pB2A1.12]
商品貨號
B242973
Designations
2A1 [Blast 1 cDNA, pB2A1, pB2A1.12]
GenBank Number

X06341

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 4.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 1.200000047683716
Tissue: lymphoblastoid cell line immortalized with EBV
Gene product: CD48 antigen(antigen CD48 (B cell membrane protein)) [BCM1]
Alleles: A1, A1, A2, A2
Insert Size (kb)
1.200
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Insert contains SacI (3), SphI (1), and HincII (1) sites.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 1.1; HincII--4.2, plus smaller; HindIII--4.4; PstI--4.4; BamHI--4.4.
The protein resulting from in vitro translation of this cDNA (unglycosylated, with signal peptide) is precipitated by MAb 17D6 specific to Blast-1.
Detects human genomic restriction fragments of the following sizes (kb): EcoRI--9.3, 6.7, 3.4 (weak); HindIII--2.9, 2.1, 1.2, 0.8.
This is a full-length or close to full-length cDNA clone including 46 bp 5' untranslated and about 430 bp 3' untranslated sequence. The sequence predicts 5 N-glycosylation sites.
The insert is oriented such that transcription from the T7 promoter gives the sense strand.
References

Lai LW, et al. A gene correcting the defect in the CHO mutant Ade-H, deficient in a branch point enzyme (adenylosuccinate synthetase) of de novo purine biosynthesis, is located on the long arm of chromosome 1. Genomics 9: 322-328, 1991. PubMed: 2004783

Fisher RC, Thorley-Lawson DA. Characterization of the Epstein-Barr virus-inducible gene encoding the human leukocyte adhesion and activation antigen BLAST-1 (CD48). Mol. Cell. Biol. 11: 1614-1623, 1991. PubMed: 1847502

Staunton DE, Thorley-Lawson DA. Molecular cloning of the lymphocyte activation marker Blast-1. EMBO J. 6: 3695-3701, 1987. PubMed: 2828034

David A Thorley-Lawson, personal communication

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