construct to generate mRNA that can be expressed in Xenopus oocytes
vector for constructing cDNA libraries
vector permitting RNA synthesis in vitro
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0; PstI--3.0; XbaI--3.0.
Cloning vector useful for in vitro transcription of cloned inserts. In vitro transcribed sequences will all contain the authentic 5'-UTR of X. laevis beta globin on the 5' end.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Dommers S, Heinlein UA. Recombinant polypeptides transplanted from pUMA vector-derived cRNAs are translocated through microsomal membranes and exported out of frog oocytes. Biochem. Biophys. Res. Commun. 204: 1346-1351, 1994. PubMed: 7980614
