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pSGP573
pSGP573
規(guī)格:
價格:
編號:B243098
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pSGP573
商品貨號
B243098
Designations
pSGP573
Depositors
J Hodson
培養(yǎng)基
Biosafety Level
1
Host
Escherichia coli HB101 (ATCC 33694)
Vector Information
Intact vector size: 9.300
Type of vector: plasmid
Cloning sites: XhoI SalI BamHI SmaI
Polylinker sites: XhoI SalI BamHI SmaI
Cloning capacity: Y
Construction: REP4X
Host range: Schizosaccharomyces pombe; Escherichia coli
Features (with orientation and position when available):
coding sequence: GFP, ->
replicon: ars1
marker(s): ampR
marker(s): ura4+
promoter for expression: nmt, ->
MCS: XhoI...SmaI, ->
Vector: pSGP573 (plasmid)
Promoters: Promoter for expression nmt
Construction: REP4X
Marker(s): ampR
Construct size (kb): 9.30
Features: marker(s): ampR
marker(s): ura4+
promoter for expression: nmt
replicon: ars1
MCS: XhoI...SmaI
coding sequence: GFP
Applications
contains sequence green fluorescent protein (GFP)
Comments
GFP sequence corresponds to Genbank Accession number U87624 with nucleotides 7-720 of GFP matching nucleotides 21-734 of U87624, with additional modification S65T by changing codon to ACC.
The order of the major features in this plasmid is: nmt promotor - XhoI - ATG - GFP - GCT - NotI - SalI - BglII - SmaI - termination sequence - ars1 - pUC119 (3.3 kb) - ura4
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
Shipped
freeze-dried
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