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pHFTA plasmid in <em>Escherichia coli</em>
pHFTA plasmid in <em>Escherichia coli</em>
規(guī)格:
價格:
編號:B243143
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pHFTA plasmid in Escherichia coli
商品貨號
B243143
Designations
pHFTA plasmid in Escherichia coli
GenBank Number

L10413

培養(yǎng)基
Species
Homo sapiens, human
Depositors
DA Andres
Vector
Total size of construct (kb): 4.6
Vector: pBluescript SK+
Vector size (kb): 2.9
Markers: ampR
Vector ends: EcoRI
Insert
Insert size (kb): 1.67
Gene: farnesyltransferase, CAAX box, alpha, FNTA
DNA: cDNA
Insert ends: EcoRI
Source: human
Genbank accession: L10413
Insert Size (kb)
1.670
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Freeze dried E. coli containing the plasmid
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 1.67; EcoRV--3.9, 0.7; SmaI--4.3, 0.3; HindIII--4.6; PstI--3.1, 0.55, 0.35 (doublet), 0.25.

Transcription from the T3 promoter gives the sense strand.
References

Andres DA, et al. cDNA cloning of the two subunits of human CAAX farnesyltransferase and chromosomal mapping of FNTA and FNTB loci and related sequences. Genomics 18: 105-112, 1993. PubMed: 8276393

Douglas A Andres, personal communication

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