ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Freeze dried E. coli containing the plasmid
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 1.67; EcoRV--3.9, 0.7; SmaI--4.3, 0.3; HindIII--4.6; PstI--3.1, 0.55, 0.35 (doublet), 0.25.
Transcription from the T3 promoter gives the sense strand.
References
Andres DA, et al. cDNA cloning of the two subunits of human CAAX farnesyltransferase and chromosomal mapping of FNTA and FNTB loci and related sequences. Genomics 18: 105-112, 1993. PubMed: 8276393
