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pBR328
pBR328
規格:
價格:
編號:B243216
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pBR328
商品貨號
B243216
Designations
pBR328
Depositors
EM Lederberg
培養基
Biosafety Level
1
Host
Distribution host: E. coli RR1
Vector Information
Size (kb): 4.9000000953674320
Vector: pBR328 (plasmid)
Promoters: Promoter none
Construction: pBR327 ori, pBR325 cml
Marker(s):ampR,cmlR,tetR
Construct size (kb): 4.900000095367432
Features: marker(s): ampR, cmlR, tetR
promoter: none
replicon: pMB1
terminator: none
Comments
A general purpose plasmid vector.
Contains a 482 bp inverted duplication. Constructed from the PstI/BamHI fragment of pBR327 containing the replication origin and the PstI/BamHI fragment of pBR325 containing the cml gene.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Soberon X, et al. Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325. Gene 9: 287-305, 1980. PubMed: 6248430

Balbas P, et al. Plasmid vector pBR322 and its special-purpose derivatives--a review. Gene 50: 3-40, 1986. PubMed: 3034735

Shipped
frozen
Shipping Information

Shipped: ?Freeze dried E. coli containing the plasmid.

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