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pCu425CUP1

規格:

價格:

編號:B243330

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pCu425CUP1

商品貨號

B243330

Designations

pCu425CUP1

Depositors

S Labbe, DJ Thiele

培養基

Biosafety Level

Vector Information

Size (kb): 7.3359999656677250

Vector: pCu425CUP1 (phagemid)

Promoters: Promoter for expression CUP1

Construction: pRS425, CUP1 promoter

Marker(s):LEU2,ampR

Construct size (kb): 7.335999965667725

Features: marker(s): LEU2
marker(s): ampR
promoter for expression: CUP1
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: SpeI...XhoI
terminator: CYC1

Applications

cloning vector

Comments

Restriction digests of the vector give the following sizes (kb): SacI--7.3; SacI/SpeI--7.0, 0.26.

Gene CUP1 is the most potent for copper induction. The CUP1 promoter can be used to creat a series of versatile vectors to allow its utilization to control the expression of heterologous genes in S.cerevisiae.

References

Labbe S, Thiele DJ. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol. 306: 145-153, 1999. PubMed: 10432452