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p11-2-2

規格:

價格:

編號:B243343

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

p11-2-2

商品貨號

B243343

Designations

p11-2-2

Species

Homo sapiens, human

培養基

Vector

Construct size (kb): 6.0

Insert

DNA: genomic

Insert lengths(kb): 0.6000000238418579

Gene product: DNA Segment, single copy [D12S6]

Alleles: A1, A1, A2, A2, C1, C2, D1, D2

Insert Size (kb)

0.600

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA distributed as 5 ug in 50 ul TE (volume: 50 ul,

Comments

Constructed by inserting Sau3A fragment of cosmid 1-11 into BamHI site.

Restriction digests of the clone give the following sizes (kb): EcoRI--5.4, 0.6; PstI--5.0, 1.0; BamHI--uncut; SalI--6.0; HindIII--6.0.

IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.

The probe hybridizes to the inserted sequence of the p11-1-111 RFLP and thus fails to hybridize to chromosomes lacking this sequence.

References

Buroker NE, et al. Four restriction length polymorphisms revealed by probes from a single cosmid map to human chromosome 12q. Hum. Genet. 72: 86-94, 1986. PubMed: 3002956

O'Connell P, et al. A primary genetic linkage map for human chromosome 12. Genomics 1: 93-102, 1987. PubMed: 3478299

Michael Litt, personal communication

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