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Shox2
Shox2
規(guī)格:
價(jià)格:
編號(hào):B243363
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Shox2
商品貨號(hào)
B243363
Organism
Mus musculus, mouse
Tissue
sinoatrial node
培養(yǎng)基
Cell Type
embryonic stem cell-derived nodal cardiac myocytes
Product Format
frozen 1.0 mL
Morphology
epithelial-like
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
embryonic
Gender
male
Strain
129S1/SVmJ
Applications
Can be used for the design of biological pacemakers, studying the biology and electrophysiology of sinoatrial nodal cells, and in animal models of cardiac pacemaker diseases such as AV block or sinus node dysfunction.
Storage Conditions
liquid nitrogen vapor phase
Images
Cell Micrograph of Shox2, Embryonic Stem Cell-derived Nodal Cardiac Myocyte, ATCC CRL-3256
Derivation
Shox2 cell originated from the genetic selection of mouse ES cells using a 3.8 kb fragment of the Shox2 promoter.
Comments
Shox2 cells originated from the genetic selection of mouse ES cells using a 3.8 kb fragment of the Shox2 promoter. They exhibit a pacemaker-like molecular phenotype and gene expression profile including pacemaker-specific transcription factors (Tbx3  Tbx5 BMP4), structural proteins (alpha-cardiac actin; alpha-skeletal actin; desmin), connexins (Cx45; Cx30.2), ion channel subunits (Cav1.2; Cav1.3; Cav3.1), a sodium-calcium exchanger (NCX1) and a hyperpolarization activated cyclic nucleoside gated channel (HCN2).  ( Hashem SI, et al. Genetic isolation of stem cell-derived pacemaker-nodal cardiac myocytes. Mol. Cell. Biochem. 383:161-171, 2013. PubMed: 23877224)
Complete Growth Medium
The base medium for this cell line is Mouse ES Cell Basal Medium, Catalog No. SCRR-2011. To make the complete growth medium, add the following components to the base medium:
  • 2-mercaptoethanol to a final concentration of 0.05 mM
  • fetal bovine serum to a final concentration of 15%

Subculturing

Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) (ATCC 30-2200) or 0.25% (w/v) Trypsin - 0.53 mM EDTA (ATCC 30-2101) solution to remove all traces of serum which contains trypsin inhibitor.
  2. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  1. Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting.
  2. Transfer cell suspension to a centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes.
  3. Discard supernatant. Resuspend the cell pellet in fresh growth medium.
  4. Add appropriate aliquots of the cell suspension to new culture vessels.
  5. Incubate cultures at 37°C.

 

Subcultivation Ratio: 1:3 to 1:6 is recommended.

Medium Renewal: 2 to 3 times a week

Cryopreservation
Freeze Medium: fetal bovine serum, 95%; DMSO, 5%
Storage Temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C; Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Volume
1.0 mL
COI

Mouse

Name of Depositor
William Claycomb, Louisana State University
Year of Origin
January 2012
References

Hashem SI, et al. Genetic isolation of stem cell-derived pacemaker-nodal cardiac myocytes. Mol. Cell. Biochem. 383:161-171, 2013. PubMed: 23877224

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