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lambda93 [GC.93]
lambda93 [GC.93]
規格:
價格:
編號:B243507
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
lambda93 [GC.93]
商品貨號
B243507
Designations
lambda93 [GC.93]
GenBank Number

L10641

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 51.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: Charon 4A
Intact vector size: 46.000
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
promoter: none
replicon: lambda
terminator: none
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GC
Genomic copy number: unique
Gene name: group-specific component (vitamin D binding protein)
Contains complete coding sequence?: N
Chromosome: 4; Localization: 4 q12-q13
Tissue: fetal liver (ATCC 37333)
Type of DNA: genomic
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert end: HaeIII or AluI Modification: EcoRI linker
Insert size (kb): 19.95
Cross references: DNA Seq. Acc.: L10641
Nucleotides ~1-19950 of the insert correspond to
nucleotides ~6300-26300 of L10641.
Insert lengths(kb): 19.95000076293945
Tissue: fetal liver (ATCC 37333)
Gene product: group-specific component (vitamin D binding protein)( vitamin D binding protein, vitamin D-binding alpha-globulin, group specific component) [GC]
Insert Size (kb)
19.950
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--19.5, 11.0, 5.0, 3.7, 2.6, 2.55, 1.9, 1.8, 0.95, 0.7; HindIII-->23, 5.9; BamHI-->23, 7.3, 5.7, 4 (doublet), 1.6;
BglII--5.5, 5.1, 4.9 (doublet), 3.5, 3.1, 2.75, 2.5, 2.15, 2.0, 1.8 (doublet), 1.7, 1.55 (doublet), 1.4, 0.65; SstI-->23, 9.0, 4.1, 3.9, 2.8, 1.9.
ATCC 95662/95663 and 95664/95665 overlap by 5. kb and share EcoRI fragments of the following sizes (kb): 0.7, 1.7.
The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--3.3, 4.0, 5.7, 9.4, 12.0, 12.6, 14.4, 14.55, 16.45, 19; HindIII--1.6; SacI--9, 11.8, 15.9.
Begins in intron 1 and continues into intron 6.
ATCC 95664/95665 and 95666/95667 overlap by 6.45 kb and share EcoRI fragments of the following sizes (kb): 0.15, 1.9, 2.5.
Contains L1 (Kpn) and Alu repeats.
References

Witke WF, et al. Complete structure of the human Gc gene: differences and similarities between members of the albumin gene family. Genomics 16: 751-754, 1993. PubMed: 8325650

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