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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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beta5 clone 5.1
beta5 clone 5.1
規格:
價格:
編號:B243556
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
beta5 clone 5.1
商品貨號
B243556
Designations
beta5 clone 5.1
GenBank Number

X16983

培養基
Species
Homo sapiens, human
Depositors
Q Yuan, ME Hemler
Vector
Construct size (kb): 6.130000114440918
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS+
Intact vector size: 2.964
Type of vector: phagemid
Vector end: EcoRI closer to XhoI site
Vector end: EcoRI
Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ITGB5
Genomic copy number: unique
Gene name: integrin, beta 5
Contains complete coding sequence?: Y
Tissue: thymus epithelium
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 3.174
Cross references:
Insert lengths(kb): 3.174000024795532
Tissue: thymus epithelium
Gene product: integrin, beta 5(integrin beta 5) [ITGB5]
Insert Size (kb)
3.174
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.1, 3.0; PstI--3.9, 1.0, 1.0, 0.2; KpnI--5.0, 0.9; PvuII--2.4, 2.1, 1.4; BamHI--6.0.
The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.64, 1.73, 1.92, 2.14; KpnI--0.92; PvuII--1.92.
References

Ramaswamy H, Hemler ME. Cloning, primary structure and properties of a novel human integrin beta subunit. EMBO J. 9: 1561-1568, 1990. PubMed: 2328726

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