DESCRIPTION OF VECTOR COMPONENT: Name of vector: SuperCos 1 Intact vector size: 7.600 Type of vector: cosmid Cloning sites: BamHI Polylinker sites: EcoRI NotI BamHI NotI EcoRI Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR, neoR, G418R promoter: T3, T7 replicon: pMB1, SV40 Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT: Genome: Saccharomyces cerevisiae Gene symbol: CDC3 Contains complete coding sequence?: Y Chromosome: XII; Localization: XII R, 225 cM from CEN Genome: Saccharomyces cerevisiae Gene symbol: REC102 Contains complete coding sequence?: Y Chromosome: XII Genome: Saccharomyces cerevisiae Gene symbol: MID2 Gene name: mating process protein Contains complete coding sequence?: Y Chromosome: XII Strain: AB972 (ATCC 76269) Type of DNA: genomic Insert end: Insert end: Insert size (kb): 37.7 Cross references:
Insert lengths(kb): 37.70000076293945
Gene product: mating process protein [CDC3]
Insert Size (kb)
37.700
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: frozen, uncharacterized
Comments
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
