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pUNG40
pUNG40
規格:
價格:
編號:B243757
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pUNG40
商品貨號
B243757
Designations
pUNG40
GenBank Number

X15653

培養基
Species
Homo sapiens, human
Applications
It does not include the complete coding region, but it does include the complete region which is conserved among uracil-DNA glycosylases.
Vector
Construct size (kb): 4.735000133514404
Insert
DNA: cDNA
Insert lengths(kb): 1.726999998092651
Tissue: placenta
Gene product: uracil-DNA glycosylase(uracil DNA glycosylase) [DGU]
Insert Size (kb)
1.727
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.0, 1.5, 0.2; EcoRI--3.0, 1.7; XbaI--4.7; HindIII--3.8, 0.9; XhoI--4.7.
The insert contains the following restriction sites (nt from 5' end[nt according to published sequence]): BamHI--15, 256 [297, 538]; SacII--33 [315]; StuI--308 [590]; NcoI--388 [670]; HindIII--850 [1132].
Gives a strong hybridization signal with genomic EcoRI fragments of 7 and 3.5 kb.
Extends from nt 283 to nt 2005 of the published sequence. It does not include the complete coding region, but it does include the complete region which is conserved among uracil-DNA glycosylases.
Oriented such that transcription from the T7 promoter gives the sense strand.
References

Olsen LC, et al. Molecular cloning of human uracil-DNA glycosylase, a highly conserved DNA repair enzyme*. EMBO J. 8: 3121-3125, 1989. PubMed: 2555154

Aasland R, et al. Chromosomal assignment of human uracil-DNA glycosylase to chromosome 12. Genomics 7: 139-141, 1990. PubMed: 2335354

Lisbeth C Olsen, personal communication

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