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pGPTV-BLEO
pGPTV-BLEO
規格:
價格:
編號:B243761
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pGPTV-BLEO
商品貨號
B243761
Designations
pGPTV-BLEO
Depositors
R Masterson, D Becker
培養基
Biosafety Level
1
Vector Information
Size (kb): 13.8000001907348600
Vector: pGPTV-BLEO (plasmid)
Construction: pBIN19, pBI101, pBIB-HYG
Marker(s):kanR
Construct size (kb): 13.80000019073486
Features: marker(s): kanR
replicon: RK2
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--13.5; BglII--8.2, 5.6; BamHI--13.5.
Vector contains the following restriction sites (approximate kb from the HindIII site): BamHI--1.6; EcoRI--11.6; SstI--11.9.
Binary vector with plant selectable marker near the T-DNA left border and a glucuronidase (uidA) reporter gene. The marker and uidA are divergently transcribed, and can be easily removed or replaced.
The order of the major features of this plasmid is: EcoRI - pAnos - SstI - uidA - XbaI - HindIII - P35S - ble - BamHI - pAg7 - T-DNA - BglII - RK2 ori - kanR.
Media
ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Becker D, et al. New plant binary vectors with selectable markers located proximal to the left T-DNA border. Plant Mol. Biol. 20: 1195-1197, 1992. PubMed: 1463855

Shipped
freeze-dried
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