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IB2329 [EST41698, SGC32142]
IB2329 [EST41698, SGC32142]
規格:
價格:
編號:B243877
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
IB2329 [EST41698, SGC32142]
商品貨號
B243877
Designations
IB2329 [EST41698, SGC32142]
GenBank Number

T15978

培養基
Species
Homo sapiens, human
Depositors
JM Sikela
Applications
The primers can also be used to generate sequence from the 3' or 5' ends, respectively.
Vector
Construct size (kb): 4.199999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, <-, 100-556
insert detection: lacZ', <-, 594-1119
primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, <-
promoter: lac, <-, 1164-1192
replicon: pMB1, <-, 1518-1518
marker(s): ampR, <-, 2276-3136
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: DNA Segment, expressed
Contains complete coding sequence?: U
Chromosome: 11; Localization: 11, 136.389999 cR
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert 5' end: Modification: HindIII
Insert 3' end: Modification: NotI
Insert size (kb): 0.9
Cross references: DNA Seq. Acc.: T15978
Insert lengths(kb): 0.8999999761581421
Tissue: infant brain, 3 months old (from Bento Soares)
Gene product: DNA Segment, expressed
Insert Size (kb)
0.900
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--3.3, 0.94; NotI--4.5; HindIII/NotI--3.4, 0.92; PstI--4.5; EcoRI--4.5.
Derived from a directionally cloned library with an average insert size of 1.7 kb.
Inserts can be amplified with the M13 universal (forward) and reverse primers. The primers can also be used to generate sequence from the 3' or 5' ends, respectively.
References

Soares MB, et al. Construction and characterization of a normalized cDNA library. Proc. Natl. Acad. Sci. USA 91: 9228-9232, 1994. PubMed: 7937745

Marcelo B Soares, personal communication

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