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HHCMG75
HHCMG75
規格:
價格:
編號:B243922
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
HHCMG75
商品貨號
B243922
Designations
HHCMG75
GenBank Number

M78038

培養基
Species
Homo sapiens, human
Vector
Construct size (kb): 4.900000095367432
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D1S282E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Sequence homology: Homo sapiens agrin
Chromosome: 1; Localization: 1
Tissue: hippocampus, female, 2 years, oligo-dT and random-primed
(Stratagene 936205)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.1
Cross references: DNA Seq. Acc.: M78038; EST: EST01625; Primer: M13 Forward
DNA Seq. Acc.: M78829; EST: EST00977; Primer: M13 Reverse
Insert lengths(kb): 2.099999904632568
Tissue: hippocampus, female, 2 years, oligo-dT and random-primed (Stratagene 936205)
Gene product: agrin [AGR*]
Insert Size (kb)
2.100
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 2.1; PstI--3.8, 1.1; BamHI--4.8.
According to the coding-region prediction program CRM, these sequence ranges (followed by the probability) of EST00977 may contain coding sequence: 182-141 (good).
EST01625 matches GenBank accession RATAGR with 84.60% identity over the sequence range: 10-113.
This sequence record is for rat agrin.
References

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

Polymeropoulos MH, et al. Chromosomal distribution of 320 genes from a brain cDNA library. Nat. Genet. 4: 381-386, 1993. PubMed: 8401586

J C Venter, personal communication

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