黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pHP45omega-Tc [JF297] B244136
pHP45omega-Tc [JF297]
pHP45omega-Tc [JF297]
規格:
價格:
編號:B244136
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pHP45omega-Tc [JF297]
商品貨號
B244136
Designations
pHP45omega-Tc [JF297]
Depositors
J Frey
培養基
Biosafety Level
1
Vector Information
Size (kb): 4.4800000190734860
Vector: pHP45omega-Tc (plasmid)
Construction: pHP45:omega, pBR322 tetR
Marker(s):ampR,tetR
Construct size (kb): 4.480000019073486
Features: marker(s): ampR, tetR
replicon: pMB1
terminator: T4D gene 32
Applications
integrating vector
shuttle vector
vector permitting positive selection for integration
vector useful for interposon mutagenesis
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.5, 2.4; HindIII--2.45, 2.3; SalI--4.9.
Because resistance levels may vary, it is important to determine the proper antibiotic concentration for each strain or isolate.
One of a series (ATCC 37653 through 37657) of vectors for in vitro mutagenesis (via the omega interposon) of gram-negative genera.
pHP45:omega was cut with BssHII + SphI (removing the spcR/strR gene) and the ends were made blunt. This was ligated to a blunt-ended 1.4 kb HindIII/AvaI fragment of pBR322 containing the tetracycline resistance gene.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Fellay R, et al. Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of Gram-negative bacteria. Gene 52: 147-154, 1987. PubMed: 3038679

Frey J, Krisch HM. Omega mutagenesis in Gram-negative bacteria: a selectable interposon which is strongly polar in a wide range of bacterial species. Gene 36: 143-150, 1985. PubMed: 2998930

Shipped
freeze-dried
  • 王經理
    手機號：17280875617
    QQ：1438578920
  • 胡經理
    手機號：13345964880
    QQ：2438244627
  • 周經理
    手機號：17757487661
    QQ：1296385441
  • 于經理
    手機號：18067160830
    QQ：2088210172
  • 沈經理
    手機號：19548299266
    QQ：2662369050
  • 李經理
    手機號：13626845108
    QQ：972239479