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pPAI75.1

規(guī)格:

價格:

編號:B244141

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pPAI75.1

商品貨號

B244141

Designations

pPAI75.1

GenBank Number

J02685

培養(yǎng)基

Species

Homo sapiens, human

Depositors

JE Sadler

Vector

Construct size (kb): 4.599999904632568

Insert

DNA: cDNA

Insert lengths(kb): 1.899999976158142

Tissue: placenta

Gene product: plasminogen activator inhibitor, type II (arginine-serpin) [PAI2]

Alleles: A1, A2, A1, A2

Insert Size (kb)

1.900

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

The insert contains the following sites separated by (bp)(approx): SalI- 280- PvuII- 60- PstI- 50- NsiI- 90- SspI- 320- XbaI- 20- NdeI- 420- NsiI- 560- XbaI- 85- SalI.

Restriction digests of the clone give the following sizes (kb): SalI--2.65, 2.0; EcoRI--2.65, 1.95; BamHI--4.65; HindIII--4.65; PstI--4.0, 0.59.

This clone includes 55 nt of 5'-noncoding sequence with a stop codon in-frame at residue -27, 1245 nt of open reading frame, a stop codon, 581 nt of 3'-noncoding sequence and a poly(A) tail.

The probe detects a single band of 2.0 kb in total mRNA from human placenta.

References

Ye RD, et al. cDNA cloning and expression in Escherichia coli of a plasminogen activator inhibitor from human placenta. J. Biol. Chem. 262: 3718-3725, 1987. PubMed: 3029122

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