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pDdeM1.6

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價格:

編號:B244249

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

斜面

甘油

平板

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產品名稱

pDdeM1.6

商品貨號

B244249

Designations

pDdeM1.6

GenBank Number

Y00449

培養基

Species

Desulfovibrio desulfuricans (Beijerinck) Kluyver and van Niel

Depositors

New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc.

Applications

produces protein modification methylase DdeI

produces protein restriction endonuclease DdeI

Vector

Construct size (kb): 6.199999809265137

Insert

DNA: genomic

Insert lengths(kb): 2.299999952316284

Gene product: modification methylase DdeI [hsdM]

Target Gene: modification methylase DdeI, restriction endonuclease DdeI

Insert Size (kb)

2.300

Media

LB plus ampicillin (100 mcg/ml) plus chloramphenicol (30 mcg/ml)

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of pDdeM1.6 give the following sizes (kb): BamHI--5.2, 1.3; BglII--6.6; ClaI/HindIII--4.4, 1.9; EcoRI--6.6; HindIII--6.4.

Restriction digests of pDdeR2.3 give the following sizes (kb): BamHI--4.4, 2.25; BglII--6.6; ClaI/HindIII--5.2, 1.4; EcoRI--6.6; HindIII--5.4, 1.4.

The pDdeM1.6 insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.8; BglII--1.0.

The 2.3 kb BamHI fragment contains all of the endonuclease and part of the methylase. Both are transcribed in the same direction.

pDdeM1.6 encodes a truncated methylase that lacks 33 amino acids from the carboxy terminus and that are replaced by 6 amino acids from pBR322. This protein shows higher activity and stability than the wild type product.

Avoid overgrowth and storage of cultures.

References

Brooks JE, O'Donnell KH. Method for producing the DDEI restriction endonuclease and methylase. US Patent 5,354,680 dated Oct 11 1994

Howard KA, et al. Cloning the DdeI restriction-modification system using a two-step method. Nucleic Acids Res. 14: 7939-7951, 1986. PubMed: 3022241

Sznyter LA, et al. Nucleotide sequence of the DdeI restriction-modification system and haracterization of the methylase protein. Nucleic Acids Res. 15: 8249-8266, 1987. PubMed: 2823226

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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