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ACY1-E

規格:

價格:

編號:B244623

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

ACY1-E

商品貨號

B244623

Designations

ACY1-E

Species

Homo sapiens, human

培養基

Depositors

YE Miller

Vector

Construct size (kb): 4.099999904632568

Insert

DNA: cDNA

Insert lengths(kb): 1.384999990463257

Tissue: liver

Gene product: aminoacylase I(aminoacylase, aminoacylase 1) [ACY1]

Insert Size (kb)

1.385

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.4; PstI--3.1, 0.8, 0.15; HindIII--4.1; BamHI--4.1; SalI--4.1.

The insert contains the following restriction sites (nt from the 5' end): PstI--716, 875; MboI--1025.

The insert is oriented in the vector so as to generate a fusion protein.

Detects a genomic EcoRI fragment of 22 kb mapping to chromosome 3 and a genomic EcoRI fragment of 4.4 kb mapping to chromosome 18. The latter fragment is less intensely visualized in both EcoRI and KpnI digests.

Detects a major 0.9 kb mRNA and a minor 4.2 kb mRNA in human liver, fibroblasts, and a variety of cell lines. Also detects a 0.9 kb mRNA in the K1 Chinese hamster ovary cell line.

References

Miller YE, et al. Human aminoacylase-1: cloning, regional assignment to distal chromosome 3p21.1, and identification of a cross-hybridizing sequence on chromosome 18. Genomics 8: 149-154, 1990. PubMed: 1707030

York E Miller, personal communication

B186400：ACY1-E

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