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Rotavirus A
Rotavirus A
規(guī)格:
貨期:
編號(hào):B244645
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) Rotavirus A
商品貨號(hào) B244645
Classification Reoviridae, Rotavirus, Rotavirus A
Deposited As Human rotavirus
Agent Rotavirus A
Strain DS-1
Applications
Enteric Research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
human infant, Bethesda, MD
Product Format frozen
Storage Conditions -20°C to -70°C?
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Comments
may cause gastroenteritis in infants
-20C to -70C
Propagate in cells fed with serum-free medium containing 0.5 ug/ml purified trypsin.
Effect on Host
Yes, in vitro effects: Cytopathic effects (rounding and detachment of cells, plaque formation) in MA-104 cells with massive cell destruction in 5 to 6 days
Vague CPE characterized by rounding and clumping of cells. Cell detachment and the appearance of floating cells in 3-6 days.
Recommended Host
MA-104 cells (ATCC CRL-2378)

Host of Choice:? MA-104 (CRL-2378)

Host Range: MA-104, CV-1

MA-104 cells (ATCC CRL-2378); CV-1 cells (ATCC CCL-70)
Growth Conditions
Temperature: 36.0°C
Duration: 1-4 days at 36C
Cryopreservation
Storage temperature: -20C to -70C
Name of Depositor Children's Hospital of Philadelphia, Wistar Institute of Anatomy and Biology
U.S. Patent
U.S. Patent Number
Special Collection NCRR Contract
Chain of Custody
ATCC <<--Children's Hospital of Philadelphia, Wistar Institute of Anatomy and Biology<<--Y. Hoshino
Source
human infant, Bethesda, MD
References

Clark HF, et al. Rotavirus reassortant vaccine. US Patent 5,626,851 dated May 6 1997

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