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當前位置: 首頁 > ATCC代理 > pKLApoRM11-6 [pKLApoIRM11-6] B244687
pKLApoRM11-6 [pKLApoIRM11-6]
pKLApoRM11-6 [pKLApoIRM11-6]
規格:
價格:
編號:B244687
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pKLApoRM11-6 [pKLApoIRM11-6]
商品貨號
B244687
Designations
pKLApoRM11-6 [pKLApoIRM11-6]
Species
Arthrobacter protophormiae (Lysenko) Stackebrandt et al.
培養基
Depositors
New England Biolabs, Inc.
Applications
in suitable host, produces protein modification methylase ApoI
in suitable host, produces protein restriction endonuclease ApoI
Vector
Construct size (kb): 9.2
Insert
DNA: genomic

Insert information:

Insert size (kb): 4.8

Gene : restriction endonuclease ApoI, modification methylase ApoI

DNA : genomic

Source : Arthrobacter protophormiae ATCC 55228

Insert ends : BglII

Insert lengths(kb): 4.80
Gene product: restriction endonuclease ApoI [hsdM]
Target Gene: modification methylase ApoI, restriction endonuclease ApoI
Insert Size (kb)
4.800
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.8, 2.5, 1.5, 0.5; HindIII--6.7, 2.5.
The insert contains the following restriction sites (approximate kb from one end): PstI--1.25, 1.75, 3.6; BamHI--1.83, 2.6, 2.8, 4.5; HindIII--2.08.
Contains and expresses the complete methylase and endonuclease genes.
Distributed in aliquots of 200 - 250 ng (20-25 ng/ul).
References

Polisson C, et al. Novel type II restriction endonuclease, ApoI, obtainable from Arthrobacter protophormiae and a process for producing the same. US Patent 5,200,337 dated Apr 6 1993

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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