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CHLA-03-AA
CHLA-03-AA
規(guī)格:
價(jià)格:
編號(hào):B244711
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
CHLA-03-AA
商品貨號(hào)
B244711
Organism
Homo sapiens, human
Tissue
Brain (Temporal lobe)
培養(yǎng)基
Product Format
frozen
Morphology
rounded-floating or loosely attached (neuronal-like)
Culture Properties
mixed, adherent and suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
anaplastic astrocytoma
Age
9 years
Gender
Female
Storage Conditions
liquid nitrogen vapor phase
Images
CRL-3035 Micrograph
Derivation
CHLA-03-AA was established from a 9 year old girl with recurrent anaplastic astrocytoma (AA). Cells were isolated from freshly resected brain tumor and were cultured in neurobasal medium after mechanical disruption.
Comments

Cultures grow as floating aggregates of round cell clusters and as lightly attached neuronal-like cells.

This cell line has been continuously carried in culture for over 18 months, and in neurobasal medium grows as spheres of varying sizes.

The generation of this cell line was made possible with the support of the Michael Hoefflin Foundation for Children's Cancer to Children’s Hospital Los Angeles, with the goal of making pediatric brain tumor lines available to the research community.

Complete Growth Medium
DMEM:F12 Medium (ATCC 30-2006) with 20 ng/mL human recombinant EGF, 20 ng/mL human recombinant basic FGF, and B-27 Supplement (Invitrogen, Cat. No.17504) to a final concentration of 2% (v/v)
Subculturing
Cultures can be maintained by the addition of fresh medium when there are numerous, healthy-appearing clusters present in suspension and pH of medium has decreased. Alternatively, subculture by gently dislodging the cells in the spent culture medium by pipetting across the monolayer or by rapping the side of the flask sharply with the palm of your hand (the latter is only preferable when working with larger flasks), followed by centrifugation with subsequent resuspension in ¼ volume of the conditioned medium and ¾ volume fresh medium.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended.
Medium renewal: Add fresh medium twice weekly (depending on cell density).
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile

D5S818: 11
D13S317: 10, 11
D7S820: 9, 10
D16S539: 11, 12
vWA: 16, 18
TH01: 6, 9
Amelogenin: X
TPOX: 8, 9
CSF1PO: 10
Name of Depositor
A Erdreich-Epstein, Saban Research Institute, Children’s Hospital Los Angeles
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