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Escherichia coli (Migula) Castellani and Chalmers
Escherichia coli (Migula) Castellani and Chalmers
規(guī)格:
價格:
編號:B244822
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Escherichia coli (Migula) Castellani and Chalmers
商品貨號
B244822
Classification
Enterobacteriaceae, Escherichia
Strain Designations
TAP56
培養(yǎng)基
Application
transformation host
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen
Storage Conditions
Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol®
no
Genotype
leu thi hsdR rpsL supE44 pro+ nad+ lac(deltaU169) galK(Am) lambdakil cI857deltabioA
Type Strain
no
Comments
Host containing defective prophage for use with vectors regulating expression from the lambda PL promoter. Encodes cI857 and a functional N gene.
Phenotype verified: galK(am), strR, leu, thi.
Because of the temperature sensitive repressor, heat shock for CaCl2 transformations should be at 32C for 10 min, followed by 1 min on ice. Add LB and grow at room temperature for 1-2 hours.
Selection for ampR is on LB + amp (100 ug/ml) plates incubated at 30-32C.
An isogenic host without N gene function is TAP106, ATCC 47075.
Medium
ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 30.0°C
Name of Depositor
TA Patterson
References

Cheng X, Patterson TA. Construction and use of lambda PL promoter vectors for direct cloning and high level expression of PCR amplified DNA coding sequences. Nucleic Acids Res. 20: 4591-4598, 1992. PubMed: 1408761

Patterson TA, et al. Improved bacterial hosts for regulated expression of genes from lambda PL plasmid vectors. Gene 132: 83-87, 1993. PubMed: 8406046

Thomas A Patterson, personal communication

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