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pIB16.12
pIB16.12
規格:
價格:
編號:B244932
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pIB16.12
商品貨號
B244932
Designations
pIB16.12
GenBank Number

M63838

培養基
Species
Homo sapiens, human
Depositors
JA Trapani
Vector
Construct size (kb): 4.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSP65
Intact vector size: 3.010
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: SP6
replicon: pMB1
terminator: none
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IFI16
Other gene symbols: IFNFIP1
Genomic copy number: unique
Gene name: interferon, gamma-inducible protein 16
Contains complete coding sequence?: U
Chromosome: 1; Localization: 1 q22
Tissue: T lymphocyte, activated
Type of DNA: cDNA
Insert 5' end: Modification: linker
Insert 3' end:
Insert size (kb): 1.53
Cross references: DNA Seq. Acc.: M63838
Insert lengths(kb): 1.529999971389771
Tissue: T lymphocyte, activated
Gene product: interferon, gamma-inducible protein 16(interferon gamma-inducible protein 16) [IFI16]
Insert Size (kb)
1.530
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.5; HindIII--3.4, 1.1; PstI--3.0, 1.5; PvuII--2.5, 1.2, 0.7; SacI--4.5.
The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.32; HindIII--0.79; PvuII--1.29.
Corresponds to nt 403-1933 of the published sequence (encoding aa 135-644).
Detects a transcript of 2.7 kb in lymphoid cells.
References

Trapani JA, et al. A novel gene constitutively expressed in human lymphoid cells is inducible with interferon-gamma in myeloid cells. Immunogenetics 36: 369-376, 1992. PubMed: 1526658

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