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pSTMV5
pSTMV5
規格:
價格:
編號:B245010
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pSTMV5
商品貨號
B245010
Designations
pSTMV5
GenBank Number

M25782

培養基
Species
satellite tobacco mosaic tobamovirus (STMV)
Depositors
G Kurath
Applications
Linearize with NsiI and transcribe with T7 RNA polymerase to produce a sense strand.
Vector
Construct size (kb): 4.300000190734863
Insert
DNA: cDNA
Insert lengths(kb): 1.059000015258789
Insert Size (kb)
1.059
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): XbaI/SphI--3.4, 1.15; SalI--4.4; BglII--4.4.
The insert contains the following restriction sites (bp from the 5' XbaI site): DraI--321; SalI--602; BglII--791. The primer-derived NsiI site is at nt 1059.
Clones pSTMV5 and pSTMV6 (ATCC 45135, 45136) can be distinguished by RNase protection assays which give distinct patterns due to an A-C difference at nt 751 of the STMV sequence.
The RNA transcribed from this clone is infectious.
Contains a complete STMV genome representing the major genome type within the STMV type strain.
pSTMV5 is identical to the reported STMV type strain sequence except for a G to A difference at nt 494.
Linearize with NsiI and transcribe with T7 RNA polymerase to produce a sense strand.
Constructed using primers for cDNA synthesis that added NsiI and SphI sites at the 3' end and an XbaI site at the 5' end.
Classification
Tobamovirus
References

Kurath G, et al. Analysis of genetic heterogeneity within the type strain of satellite tobacco mosaic virus reveals several variants and a strong bias for G to A substitution mutations. Virology 189: 233-244, 1992. PubMed: 1604813

Mirkov TE, et al. Nucleotide sequence and translation of satellite tobacco mosaic virus RNA. Virology 170: 139-146, 1989. PubMed: 2718378

Gael Kurath, personal communication

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