Bacteriological peptone 10g Glucose 10g Yeast extract 2.0 g Ox bile, desiccated 8.0g Glycerol 10mL Glycerol monostearate 0.5g Tween 60 5.0mL Olive oil 20mL Agar 15g Deionized water 1.0 L Medium is sterilized by autoclaving at 110C for 20 minutes. For liquid media, omit the agar.