培養基配方
Agar Medium
Nutrient Agar (BD 213000)…………………..23 g
DI Water………………………………….……1000 ml
Autoclave at 121oC.
Broth Medium
Nutrient Broth (BD cat 234000)…………….8.0 g
DI Water…………………………………..….1000 ml
Autoclave at 121oC.
*This product is commercially available from BD.
To make medium from scratch, follow formulation below:
Nutrient Agar Composition
Beef Extract……………………..…………..3.0 g
Peptone……..…………………….…………5.0 g
Agar………………………………………….15.0 g
Final pH 6.8 +/- 0.2.
*Omit agar for broth medium.
