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Solution A (see below) 0.5 ml
Solution B (see below) 0.5 ml
Solution C (see below) 1.0 ml
Solution D (see below) 0.5 ml
Solution E (see below) 0.5 ml
Solution F (see below) 2.0 drops
DI Water to 1000 ml
Autoclave at 121oC.
Solution A
CaCl2 2.0g
DI Water 100.0 ml
Solution B
MgSO4 . 7H2O 20.0 g
DI Water 100.0 ml
Solution C
EDTA 0.14 g
FeSO4 . 7H2O 0.5 g
H2SO4 (conc.) 0.05 ml
DI Water 100.0 ml
Solution D (trace metals)
Na2MoO4 . 2H2O 0.1 g
MnCl2 . 4H2O 0.2 g
CoCl2 . 6H2O 0.002 g
ZnSO4 . 7H2O 0.1 g
CuSO4 . 5H2O 0.02 g
DI Water 1000 ml
These ingredients should be dissolved separately and then added together to make 1000 ml.
Solution E
NaNO2 41.4 g
DI Water 100.0 ml
Solution F
K2HPO4 1.74 g
DI Water 100.0 ml

250g/瓶

常溫

3年